Figure 3
From: Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation
Colocalization of dysbindin-1B with aggresomal proteins. (A–D) COS1 cells were transfected with GFP-tagged control or dysbindin-1A, -1B and -1C (a, d, g, j of A–D; green) and immunostained with antibodies against γ-tubulin, 20S proteasomal subunit, or vimentin (b, e, h, k of A–C; red). The merged confocal images demonstrate colocalization of GFP-tagged dysbindin-1B with the two aggresome markers (c, f, i, and l of A and B; merged). The intermediate filament vimentin was surrounded with the aggregates (i of C). The arrows mark aggregates. (D) GFP-tagged dysbindin-1A, -1B or -1C expressing COS1 cells (a, d, g, j; green) were stained with a lysosomal marker, LysoTracker (b, e, h, k; red). Aggregates of GFP-tagged dysbindin-1B (arrow) were not colocalized with LysoTracker signals (c, f, i, l; merged). Scale bar, 20 μm.
