Figure 4
From: Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation
Different sensitivities of dysbindin-1A and dysbindin-1B to MG132 treatment and deletion of the PEST domain in dysbindin-1A results in aggregate formation. (A) The proportion of the COS1 cells containing aggregates 24, 48, and 72 h after transfection. (B) The proportion of the COS1 cells containing aggregates after transfection with 0.5–4 μg plasmids. (C) The levels of soluble myc-tagged dysbindin-1A and -1B in the absence or presence of MG132. (D) The ratio of soluble myc-tagged dysbindin-1A and -1B between MG132 treated and untreated cells. (E) The proportion of the MG132-treated COS1 cells containing aggregates. Scale bar, 20 μm. (F) Expression of dys1AΔPEST-myc deletion mutant in mouse cortical neurons leads to aggregate formation (arrow). (G) Quantification of the percentage of cells containing aggregates in mouse cortical neurons expressing myc-tagged dysbindin-1A, -1B and dysbindin-1AΔPEST. Scale bar, 40 μm. Data are presented as mean±s.e.m. *P<0.05,***P<0.001. ns, not significant.
