Figure 5
Reduced severity of colitis in miR-301a−/− mice correlates with inhibition of NF-κB and Stat3 activation and downregulation of cytokine expression. (a) Colon sections were stained using antibodies against phospho-Stat3 and phospho-p65 to evaluate activated NF-κB and Stat3 (n=10 per group). Scale bars, 50 μm. (b) Western blot analysis of colon tissue lysates for NF-κB and Stat3 activation. (c) Cytokine gene expression in colon tissues of WT and miR-301a−/− mice as determined by qPCR (n=3 for the untreated group and n=5 for the DSS-treated group). (d–f) IL-6 (d), TNF-α (e), and IL-17 (f) levels in the serum of WT (n=5) and miR-301a−/− (n=5) mice as determined by ELISA. (g–i) Levels of IL-6 (g), TNF-α (h), and IL-17 (i) secreted from colon ex vivo as determined by ELISA. Values are means±s.d. *P⩽0.05. **P⩽0.01 indicates the significance of the differences between the indicated groups.
