Figure 6
Deletion of miR-301a in hematopoietic cells leads to reduced severity of DSS-induced colitis and colitis-associated colon cancer development. WT→WT, WT mice transplanted with bone marrow from WT mice; miR-301a−/−→WT, WT mice transplanted with bone marrow from miR-301a−/− mice (n=5 each). (a) Body weight during DSS-induced colitis in chimeric mice. (b) Overview of colon shortening in DSS-treated mice. (c) Colon length in mice treated with DSS. (d) Representative histological sections of mouse colon stained with hematoxylin and eosin (H&E). Scale bars, 200 μm. (e) Colitis severity score. (f) Western blot analysis of p65 and Stat3 to determine NF-κB and Stat3 activation. (g) H&E histological sections of colon tumors from chimeric mice treated with AOM/DSS. Scale bars, 500 μm (left) and 200 μm (right). (h–j) Bar graphs showing tumor number (h), tumor size (i), and tumor size distribution (j) in AOM/DSS-treated mice. Values are means±s.d. *P⩽0.05. **P⩽0.01 indicates the significance of the differences between the indicated groups.
