Figure 5
From: An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology
The RAMP1 ECD influences the dynamics of the CTR ECD. (a) Comparison of RMSF of the principle eigenvector for CTR alone and in complex with RAMP1. (b) Movement of the cα atoms of the CTR alone (red) with CTR (blue) in complex with RAMP1 (pink) showing the midpoint and extreme fluctuations of the principle eigenvector. (c) X-stick representation of the key residues for peptide binding for the CTR alone (blue) or in the presence of RAMP1 (pink), with W84 of RAMP in off-white. Residues in loop 5 (H121, R126 and W128) and in loop 2 (F99, D101 and F102) are substantially displaced when RAMP1 interacts with the receptor, and this is consistent with the MD simulations. (d–f) display the reorganization of the surface of the peptide-binding pocket; (d) CTR alone; (e) CTR in the presence of RAMP1; and (f) CTR in the presence of RAMP1 displaying the modelled amylin peptide (blue).
