Figure 8
RNF126 promotes tumorigenicity in a PDK-dependent manner. (a) Western blot analysis of exogenous PDK1 expression in control and RNF126-depleted MDA-MB-231 cells. (b and c) Control and RNF126-depleted MDA-MB-231 cells expressing exogenous PDK1 were cultured under attached or detached conditions, after which they were assayed to determine their oxygen consumption rates (b) and cellular ATP contents (c). (d) The number of colonies formed in soft agar by control and RNF126-depleted MDA-MD-231 cells expressing exogenous PDK1. The diameter (ø) of the counted colonies is indicated as the average±s.d. (e) Tumor growth following subcutaneous implantation of control and RNF126-depleted MDA-MB-231 cells expressing exogenous PDK1 in mice. In (b–d), the error bars indicate the s.d. (n=3). The data were analyzed using a t-test. **P<0.01. NS, not significant. The data shown are representative of three independent experiments with similar results. In (e), the error bars indicate the s.e.m.; n=8 from two independent experiments (n=4 and n=4, respectively); the data shown were analyzed using the Mann–Whitney U-test. **P<0.01. RFU, relative fluorescence units.
