Figure 1

Binding of Hop to Hsp90. Increasing molar ratios of (Hsp90)2:Hop (1 μm:100 nm to 1 μm) from bottom to top lead to formation of the (Hsp90)2Hop complex. The lower spectrum is recorded in the absence of Hop. As the concentration of the free Hsp90 dimer is depleted (blue) by addition of Hop, the population of monomeric Hsp90 (green), is reduced. Inset: plot of the percentage signal intensity for monomeric Hsp90 against the concentration of Hop in solution. The percentage abundance was calculated from Massign [22].