Figure 3

Formation of the hexameric client complex. (a) Incubation of stoichiometric ratios (all at 0.5 μm) of (Hsp70)2, GR, Hop and (Hsp90)2 in the presence of Hsp40 leads to the formation of a hexameric client-transfer complex as reported previously [18]. (b) On addition of an equimolar ratio of FKBP52 to this complex binding of a single FKBP52 subunit is observed (charge states labelled blue).