Figure 5 | Cell Discovery

Figure 5

From: The interchange of immunophilins leads to parallel pathways and different intermediates in the assembly of Hsp90 glucocorticoid receptor complexes

Figure 5

Formation of a client-transfer complex containing FKBP51 and subsequent challenge with p23 or FKBP52. (a) Incubation of an excess of FKBP51 in a solution containing Hsp902, Hsp702, Hop, GR (all at 1 μm), Hsp40 and ATP leads to the formation of the client-transfer complex (Hsp90)2(Hsp70)2(FKBP51)1(GR)1. (b) Following challenge of the client-transfer complex with p23 the predominant complex is (Hsp90)2(GR)1(FKBP51)1(p23)2. (c) Subsequent exchange of FKBP51 for FKBP52 leads to the formation of a series of complexes including (Hsp90)2(GR)1(FKBP52)1 the nuclear transfer complex and the client-transfer complex with exchange of FKBP51 for FKBP52. Interestingly no (Hsp90)2(GR)1(FKBP51)1 is formed in the presence of p23. A full assignment of this spectrum is available in Supplementary Figure S8.

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