Figure 4

Photosynthetic electron flow is disrupted in pbr1-1. (a) Phenotypes of 16-day-old plants of the wild-type (WT), pbr1-1 and PBR1 overexpression lines (OE-5 and OE-7) grown in greenhouse. (b) Expression levels of PBR1 in the WT and PBR1 overexpression lines (OE-5 and OE-7) analyzed by quantitative reverse transcriptase–PCR. (c) Measurements of photosynthetic parameters in leaves of the indicated genotypes as shown in a. The plants were exposed to very bright saturating pulses of light (flashing once every 30 s in 5 min), and fluorescence images were shown at the first, second, fifth and tenth flash (from left to right). Fluorescence was measured with CF Imager and visualized using a pseudocolor index, as indicated on the right.