Figure 1

Reduced body mass, increased energy expenditure and improved insulin sensitivity in DJ-1 knockout mice during aging and high-fat diet. (a) Body mass of wild-type (WT) and DJ-1 knockout male mice (KO) fed on a chow diet for 40 weeks (WT, n=10; KO, n=10). (b) Body lean and fat mass of 12-month-old mice measured by MRI (WT, n=7; KO, n=6). (c) Histology of adipose tissue from 12-month-old mice. eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue; BAT, brown adipose tissue. (d) Statistical analysis energy expenditure (EE) by General Linear Model (GLM) and ANCOVA analysis of the 6-month-old male DJ-1 KO and WT mice (n=8 per genotype). (e, f) Quantification of the area under the curve (AUC) of GTT (e) and ITT (f) indicated age (n=6 per genotype). (g) Body mass of mice that were fed with a high-fat diet (HFD) (WT, n=14; KO, n=13). (h) Statistical analysis by General Linear Model (GLM) and ANCOVA analysis of the HFD induced indicated genotype mice energy expenditure (EE) (WT, n=13; KO, n=10). (i) GTT was performed in HFD-fed mice with intraperitoneal injection (IP) 2 g kg⁻¹ glucose after fasting 16 h (WT, n=6; KO, n=8).