Figure 3
From: DJ-1 maintains energy and glucose homeostasis by regulating the function of brown adipose tissue
DJ-1 maintains BAT function. (a) Gene expression in BAT from 12-month-old WT and DJ-1 KO mice (n=6 per genotype). (b) Gene expression in BAT from 4-week-old WT and DJ-1 transgenic mice (WT, n=6; TG, n=7). (c) Ucp1 immunostaining of BAT from indicated genotype mice, upper panel tissues from 12-month-old mice and bottom panel tissues from 6-month old. (d) Body mass of mice with sham operation, BAT transplantation from wild-type (WT BAT) and DJ-1 KO (KO BAT) mice (sham, n=11; WT BAT, n=13; KO BAT, n=8). (e) HE (upper panel), Ucp1 immunostaining (middle panel) and immunoblotting (bottom panel) of endogenous BAT from sham operation, WT BAT transplantation and KO BAT transplantation mice. (f) GTT assays of 19-week-old and HFD-induced recipient mice (sham, WT BAT or DJ-1 KO BAT). (g) Body mass curve of the receiver mice of WT BAT or Tg BAT transplantation or sham (n=6 per group). WT BAT or Tg BAT was transplanted subcutaneously with the BAT from wild type or DJ-1 transgenic mice. (h) HE (upper panel), Ucp1 immunostaining (middle panel) and immunoblotting (bottom panel) of the endogenous BAT from the groups indicated. (i) GTT assay results for 25-week-old DJ-1 transgenic mice transplanted with BAT. (Data are shown as the mean±s.e.m. NS, non significant, *P<0.05, **P<0.01, ***P<0.001 in comparison with sham, †P<0.05, ††P<0.01 in comparison with WT BAT, determined using the two-tailed Student’s t-test).
