Figure 6
From: A CRISPR-based approach for targeted DNA demethylation
The non-additive effect of upregulating gene expression in dCas9-based demethylation system. (a) The non-additive effect of R3 and R8 sgRNAs-guided demethylation in activation of mRNA transcription of human RANKL gene in HEK-293FT cells, in comparison with that of single sgRNA (R3 or R8). (b) The non-additive effect of M1-7 in comparison with prominent single sgRNAs (the same results showed in Figure 5b) in HeLa cells. n.s., not significant from unpaired t-test. Data were shown after normalization to the controls (blank group) (means±s.e.m., n=3).
