Figure 2 | Cell Discovery

Figure 2

From: MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300

Figure 2

Characterization of the histone crotonyltransferase (HCT) activity of MOF and its yeast homolog Esa1. (a) Immunofluorescent (IF) assay showing that MOF can catalyze histone crotonylation at multiple sites. HeLa cells were transfected with Flag-MOF and IF assays were performed with site-specific crotonylated histone antibodies as indicated. (b) Western blotting (WB) analysis showing that MOF can catalyze histone crotonylation at multiple sites. (c) The phylogenetic tree of mammalian MOF and its related yeast histone acetyltransferase (HAT) proteins. (d) WB analysis of core histones derived from the wild-type, Esa1(E338D), sas2∆ and sas3∆ yeast cells. Note the substantially reduced levels of crotonylation and acetylation in core histones derived from Esa1(E338D) mutant but not sas2∆ and sas3∆ cells. Also shown were core histones revealed by ponceau S staining. (e) Sodium crotonate treatment resulted in substantially increased levels of histone crotonylation in the wild-type but not Esa1(E338D) mutant cells. Yeast cells were treated with an increasing concentration of NaCr for 4 h and core histones were prepared and subjected to WB analysis using pan-crotonylated lysine, pan-acetylated lysine or H4 antibody as indicated.

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