Figure 4 | Cell Discovery

Figure 4

From: MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300

Figure 4

Identification of novel CBP and p300 mutants with deficient histone acetyltransferase (HAT) and competent histone crotonyltransferase (HCT) activity. (a) Diagram illustrating the acetyl-coA binding pocket of p300. PDB 3BIY. (b) The acetyl-coA binding pocket sequence alignment between CBP and p300. (c) Immunofluorescent (IF) staining assay showing that the p300 I1395G mutant was inactive for acetylation but active for crotonylation. Scale bar, 20 μm. (d) IF staining assay showing that the CBP I1432G mutant was inactive for acetylation but active for crotonylation. Scale bar, 20 μm. (e) Western blotting (WB) analysis of core histones derived from WT or I1432G mutant CBP expressed 293T cells. Lanes 2 and 4, 2 μg plasmids; lane 3 and 5, 4 μg plasmids. (f) WB analysis of core histones derived from WT or I1395G mutant p300 expressed 293T cells. Lanes 2 and 4, 2 μg plasmids; lane 3 and 5, 4 μg plasmids.

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