Figure 6

Both p300 I1395G and CBP I1432G mutants are functionally active transcriptional coactivators. (a) The p300 I1395G mutant but not the I1395R mutant enhanced transcriptional activation by SMAD3 in lucliferase reporter assay. Data are represented as relative luciferase activity (×10³)±s.d. of three technical duplicates. The expression of transfected Flag-SMAD3 and wild-type and p300 mutants was revealed by western blotting (WB) analysis. (b) The p300 I1395G mutant but not the I1395R mutant enhanced transcriptional activation by liganded AR in lucliferase reporter assay. Data are represented as relative luciferase activity (×10⁴)±s.d. of three technical duplicates. The cells were treated with 100 nm dihydrotestosterone (DHT) overnight before being collected for luciferase assay. (c) The CBP I1432G mutant but not the R1664H mutant enhanced transcriptional activation by SMAD3 in lucliferase reporter assay. Data are represented as relative luciferase activity (×10³)±s.d. of three technical duplicates. (d) The CBP I1432G mutant but not the R1664H mutant enhanced transcriptional activation by liganded AR in lucliferase reporter assay. The cells were treated with 100 nm DHT overnight. Data are represented as relative luciferase activity (×10⁴)±s.d. of three technical duplicates. All statistical analysis was performed using SigmaPlot 12.5.