Figure 1
Programmable gene repression by ddCpf1/crRNA. (a) ddCpf1-mediated repression of the transcription lacZ in MG1655. Positions of the crRNAs and sgRNAs designed for lacZ were illustrated, targeting to either the T strand or the NT strand of lacZ. Although only the crRNAs targeting to the T strand showed remarkable repression. The transcription of lacZ in cells expressing ddCpf1 alone was employed as a control, and the value was normalized to 1000. Two sgRNAs were also designed, and the dCas9-mediated repression was more effective with sgRNA targeting to the NT strand, which could be found in Supplementary Figure S1. (b) ddCpf1-mediated repression of the transcription malT in MG1655. crRNAs were designed to target both the promoter region and the T strand in the coding region of malT, and the guide sequences could be found in Supplementary Table S1. The transcription of malT in cells expressing ddCpf1 only was employed as a control, and the value was normalized to 1 000. Symbols of malT-T, malT-TP and malT-NTP represented crRNAs targeting to the T strand in the coding region, T strand in the promoter region and the NT strand in the promoter region of malT gene, respectively.
