Figure 4
Multiplex gene repression with ddCpf1 and a crRNA array. Four target genes (malT, proP, degP and rseA) were analyzed, employing the non-target rpoE gene as an internal control. The order of the target genes was shown, which differed in array 1 from array 2. The transcriptional level of each gene was analyzed in cells expressing ddCpf1 with either individual crRNA or crRNA arrays, and cells expressing ddCpf1 only were employed as a control. For rpoE gene, its transcriptional level was analyzed in cells individually expressing all the tested crRNAs, and its value in cells expressing ddCpf1 only was normalized to 1000.
