Figure 4
From: miRNA-200c-3p is crucial in acute respiratory distress syndrome
NF-κB signaling pathway mediates the upregulation of miR-200c-3p. (a) A549 cells were transfected with the corresponding siRNAs and then challenged with AF or H5N1 (MOI=4) for 48 h. The expression of miR-200c-3p was detected by qRT-PCR. (b) A549 cells were treated with CAPE or JSH-23 and then challenged with AF or H5N1 (MOI=4) for 48 h. The expression of miR-200c-3p was detected by qRT-PCR. (c) A549 cells were transfected with the corresponding siRNAs and then transfected with 1 μg ml−1 RNA extracted from AF- or H5N1-infected cells for 12 h. The expression of miR-200c-3p was detected by qRT-PCR. (d) A549 cells were treated with CAPE or JSH-23 and then transfected with 1 μg ml−1 RNA extracted from AF- or H5N1-infected cells for 12 h. The expression of miR-200c-3p was detected by qRT-PCR. (e) A549 cells were transfected with the corresponding siRNAs and then transfected with 1 μg ml−1 poly (I:C) for 12 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, A549 cells were treated with the solvent used. (f) A549 cells were treated with CAPE or JSH-23 and then transfected with 1 μg ml−1 poly (I:C) for 12 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, A549 cells were treated with the solvent used. (g) THP1 cells were transfected with the corresponding siRNAs and then treated with 1 μg ml−1 LPS for 24 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, THP1 cells were treated with the solvent used. (h) THP1 cells were treated with CAPE or JSH-23 and then treated with 1 μg ml−1 LPS for 24 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, THP1 cells were treated with the solvent used. (i) THP1 cells were transfected with the corresponding siRNAs and then treated with 10 μg ml−1 LTA for 24 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, THP1 cells were treated with the solvent used. (j) THP1 cells were treated with CAPE or JSH-23 and then treated with 10 μg ml−1 LTA for 24 h. The expression of miR-200c-3p was detected by qRT-PCR. Native, THP1 cells were treated with the solvent used. The data are shown as the mean±s.e.m. *P<0.05 and **P<0.01.
