Figure 5
From: Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities
Metformin effects were not influenced by the status of phosphatase and tensin homolog (PTEN). (a) Colony-forming assay. Histograms showing average colony counts of BT-549 cells expressing either a control vector or a calcium-binding protein 39-like (CAB39L)-expressing vector or CAB39L- or Sestrin-1 (SESN1)-targeting shRNAs or and treated with vehicle or metformin (0.5 mM) before seeding at clonal density. Statistics (t-test): P<0.05. (b) Invasion assay. BT-549 cells transfected and treated as from panel (a) for 24 h and the number of migrated cells was scored. Statistics (t-test): P<0.05. (c, d) Clonogenic assay. Isogenic HCT116 bearing a wt PTEN (c) or a shRNA targeting PTEN (PTEN K/D) (d) were treated with vehicle or metformin (0.5 mM) and colonies stained and counted 8 days later. Histograms indicating the average±s.e. Statistics (t-test): P<0.05. (e, f) Invasion assay. The cells were treated with vehicle or metformin (0.5 mM) for 24 h and the percentage of migrated cells was scored. Histograms indicating the average±s.e. of triplicate experiments. Statistics (t-test): P<0.05.
