Figure 5
From: TRPA1 channel mediates organophosphate-induced delayed neuropathy
Pharmacological inhibition of TRPA1 alleviates OPIDN in hens. (a) Schematic representation of the administration of TOCP and malathion to hens. (b) Whole-cell currents of a HEK293 cell expressing cTRPA1 channels elicited by 100 μM TOCP in calcium-free external solution (upper) and the dose–response curve of the TOCP activation effect (lower) along with the structure of TOCP (n=4–8). (c) Time course of the changes of OPIDN scores following vehicle (black, n=6), TOCP (750 mg kg−1, red, n=8), TOCP+HC030031 (100 mg kg−1) from day 0 (blue, n=8) and from day 11 (purple, n=8). (d) Time course of the changes of the neuropathy scores of hens following vehicle (black, n=6), malathion (75 mg kg−1, red, n=8), malathion+HC030031 (100 mg kg−1) from day 0 (blue, n=8) and from day 11 (purple, n=8). The clinical symptoms of the delayed neuropathy was estimated in hens daily for 21 days using a five-point scale. (e) TEM image of hen spinal cords (upper row) and sciatic nerves (lower row) from the various groups (10 000×). For panels (c) and (d), two-way ANOVA was used for statistical analysis, *P<0.05, **P<0.01, ***P<0.001.
