Figure 2

Slc39a14^−/− mice develop Mn accumulation in a variety of tissues. (a–d) Mn (a), Fe (b), Zn (c) and Cu (d) levels were measured in the indicated tissues of wild-type (WT) and Slc39a14^−/− (KO) mice at >24 weeks of age using ICP-MS (n=4 mice/group). In a, note the break in the y-axis for serum Mn. *P<0.05, unpaired Student’s t-test. (e) Serum Mn levels in Slc39a14^−/− mice treated for four weeks with Na₂CaEDTA (370 mg/kg body weight) or saline (n=8–12 mice/group, 5 month of age). (f) Rotarod test results of Slc39a14^−/− mice treated for four weeks with Na₂CaEDTA or saline (n=8–12 mice/group, 5 month of age). In e and f, *P<0.05 and **P<0.01, one-way ANOVA with Tukey’s post hoc test.