Figure 4

Hepatic Slc39a14 regulates Mn homeostasis by controlling the uptake of Mn in the liver. (a) Gene targeting strategy for generating the hepatocyte-specific Slc39a14-knockout mice (Slc39a14^fl/fl;Alb-Cre⁺, upper panel) and Slc39a14^−/− mice (lower panel). (b) Slc39a14 mRNA levels in the liver of Slc39a14^fl/fl;Alb-Cre⁺ and control (Slc39a14^fl/fl;Alb-Cre⁻) mice (n=4 mice/group). (c, d) Summary of body weight (c) and rotarod test results (d) for Slc39a14^fl/fl;Alb-Cre⁺ and control mice (n=4 mice/group). (e–h) Levels of Mn (e), Fe (f), Zn (g) and Cu (h) were measured in the indicated tissues of Slc39a14^fl/fl;Alb-Cre⁺ and control mice at 8–12 weeks of age (n=3 mice/group). *P<0.05 and **P<0.01, unpaired Student’s t-test.