Figure 4
From: A TBX5 3′UTR variant increases the risk of congenital heart disease in the Han Chinese population
TBX5 3′UTR variant rs6489956 affected heart development in zebrafish embryo. (a–d ′) Examples of heart defects with variable severity in zebrafish: (a and a ′) normal heart; (b and b ′) mild defect: heart shows mild looping arrested, pericardium effusion, and dropsy of ventral position; (c and c ′) moderate defect: heart shows moderate looping arrested, pericardium effusion and dropsy of ventral position; (d and d ′) severe defect: malformed embryo with small, string-like heart, severe pericardium effusion, and dropsy of ventral position. Green A indicates ventricle; red V indicates atrium. (e) Distribution of four categories of heart morphologies in each experimental group at 48 hpf: WT control, MO standard control, MO (5 ng), MO (2.5 ng), MO (0.5 ng), MO+C allele mRNA, MO+T allele mRNA, MO+C allele mRNA+miR-9, MO+T allele mRNA+miR-9, MO+C allele mRNA+miR-30a, MO+T allele mRNA+miR-30a. (f) EGFP sensors were coinjected with mCherry control as indicated. miR-9/30a precursor injection reduced EGFP levels in EGFP-3′UTR sensor (third column), while mCherry levels were unchanged. In the EGFP-3′UTR-T sensor (fourth column), more significant reduction in EGFP was noted.
