Table 1 Impact of Gykl1 or Gk2 knockout on spermatozoa mitility

From: Glycerol kinase-like proteins cooperate with Pld6 in regulating sperm mitochondrial sheath formation and male fertility

Parameter

WT

Gykl1 +/−

Gykl1 −/−

Gk2 +/−

Gk2 −/−

Motile (%)

52.75±8.6

39.82±7.57

26.05±4.84***

50.91±12.79

21.12±6.27***

CurviLinear velocity (μms−1)

200.41±19.79

156.59±9.27**

132.19±20.06**

174.87±42.55

106.91±16.6***

Straight-line velocity (μm−1)

62.51±6.66

27.75±8.87***

22.67±9.33***

50.5±10.97*

24.54±10.51***

Average path velocity (μms−1)

95.59±7.56

56.48±12.05***

46.24±10.94***

81.08±19.12

43.69±13.45***

Amplitude of lateral head movement (μm)

11.37±1.17

6.62±0.81*

4.53±2.55*

8.37±2.47

4.48±2.67*

Beat-cross frequency (Hz)

6.12±0.41

4.04±1.39*

2.42±1.38**

6.71±0.73

1.97±1.95**

  1. Abbreviations: GyK, glycerol kinase; WT, wild type.
  2. Parameters of spermatozoa were analyzed by Computer-Assisted Spermatozoa Analysis system.
  3. Data are
  4. mean±s.d., n=3–7, *P<0.05, **P<0.01, ***P<0.001.
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