Figure 2
From: Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration
FOR20 inhibits microtubule polymerization in vitro. (a) Microtubules were assembled with rhodamine-labeled and unlabeled tubulin (1:9) in the presence of taxol (20 μm) and GTP (1 mm). The assembled microtubules were then added by the indicated purified protein or chemicals, and processed for confocal microscopy. Scale bar, 5 μm. His-tag, a peptide of six histidine residues; NOC, nocodazole. (b) Microtubules assembled with tubulin (17 μm) in the presence of taxol (20 μm) and GTP (1 mm) were added by the indicated purified protein or chemicals, and then measured by light scattering at 340 nm wavelength with spectrophotometer. Data are analyzed by the GraphPad Software (Inc. La Jolla, CA, USA). (c) Tubulin dimers (17 μm) were mixed with the indicated protein or chemicals in the presence of taxol (20 μM) and GTP (1 mM) and subjected to spectrophotometer analysis. Data are plotted using the GraphPad Prism 5 program.
