Figure 6
From: Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration
Depletion of FOR20 stabilizes microtubules in mammalian cells. (a) HeLa cells transfected with pBS/U6 or pBS/U6-FOR20 plasmid were lysed and subjected to western analysis. GAPDH was used as an internal control. (b) The cells with the indicated treatments were used for immunofluoresence analysis with anti-α-tubulin antibody. DNA was visualized with DAPI. Scale bar, 10 μm. (c and d) HeLa cells were treated with nocodazole (5 μm) for the indicated times and processed for immunostaining with anti-α-tubulin antibody (c). The intensity of microtubules was measured by MetaMorph software (Molecular Devices) and data are presented as mean±s.d. (d). (e–g) HeLa cells treated with nocodazole (5 μm) for 3 h and were washed out to allow microtubule regrowth for the indicated times and processed for immunofluoresence (e). The intensity (f) and astral length (g) of microtubules were evaluated by MetaMorph software. The mean fluorescence intensities of 30 cells in each group were determined, and data are expressed as mean±s.d. Scale bar, 10 μm. N.S., not significant (P>0.05); *P<0.05 and **P<0.01, student’s t-test.
