Figure 2
Removal of individual subunits impacts the organization of the telosome. (a) For each subunit of the telosome, we generated inducible Cas9 cells stably expressing two sgRNAs targeting the gene. Cells were treated with doxycycline for up to 6 days before being analyzed by western blotting using the indicated antibodies. Anti-SMC1 and -actin antibodies served as loading controls. Molecular weight makers (kDa) are indicated on the right. (b) Cells from a (after 6 days of induction) were immunoprecipitated using appropriate antibodies to bring down associated telomeric DNA for dot blotting and hybridization with a telomere repeat probe (TTAGGG)3. Signals for each cell line were quantified and normalized against input. At least three experiments with independent doxycycline inductions were performed for each cell line, and the results were combined and plotted as indicated. Error bars indicate s.e. P-values were obtained using the Student’s t-test. *P<0.05, **P<0.01.
