Figure 3 | Cell Discovery

Figure 3

From: Retinoic acid induces white adipose tissue browning by increasing adipose vascularity and inducing beige adipogenesis of PDGFRα+ adipose progenitors

Figure 3

RA activates VEGF signaling to increase PDGFRα+ adipose progenitors in vivo. C57BL6 mice were injected with RA (10 mg kg−1 BW injected every other day) for 1 week. (a) Representative images showing blood vessel and adipocytes in iWAT. (b) Quantification of blood vessel density. (c) Angiogenic gene expression in iWAT. (d) Surface temperature. (e) Rectal temperature. (f) Brown adipose gene expression in iWAT. (g) Serum triacylglycerols. (h) Serum-free fatty acids. (i) Serum glucose. (j) Proliferating (Ki67 labeled) and PDGFRα-positive cells in iWAT of WT and P-V-KO (PDGFRαCreER-VEGFR2loxP) conditional VEGFR2 knockout mice in response to 1 week of RA (10 mg kg−1 BW) or VEGF164 (2 μg kg−1 BW) injection (injected every other day, and same doses for other in vivo experiments if unstated). Data presented are mean±s.e.m., n=6, *P<0.05. Scale bar=100 μm.

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