Figure 4

RA promotes the differentiation of PDGFRα-positive cells into beige adipocytes in vivo. (a) PDGFRα tracking mice. (b) Graph showing time points of tamoxifen and RA treatment. (c) PDGFRα tracking mice were injected with tamoxifen, then injected with vehicle (DMSO) or 10 mg kg−1 BW RA 24 h later. Representative images show blood vessels (red) and PDGFRα+ cells (green) in iWAT of PDGFRα tracking mice at different time points. (d) Graph showing time points of tamoxifen and RA treatment. (e) Representative images of blood vessels and PDGFRα+ cells in iWAT of PDGFRα tracking mice injected with tamoxifen once every week. (f) Quantification of GFP+ adipocytes. Data presented are mean±s.e.m., n=6, *P<0.05. Scale bar=100 μm.