Figure 1 | Cell Discovery

Figure 1

From: Binding to RNA regulates Set1 function

Figure 1

Purified Set1C binds directly to RNA in vitro. (a) A schematic diagram of Set1 and derived fragments with predicted RRM1, RRM2, N-SET and post-SET (hatched box) domains and associated subunits. FL indicates full-length. (b) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and Coomassie blue staining of purified Set1Cs reconstituted with baculoviruses expressing FLAG-Set1 or FLAG-Set1 fragments and untagged subunits. FLAG-Set1 polypeptides are marked by asterisks. (c, d) Radiolabeled GAL1 (c) and GAL10 (d) transcripts were subjected to in vitro RNA electrophoretic mobility shift assays with 0.5 (lanes 2, 4, 6, 8, 10 and 12) or 2.5 (lanes 3, 5, 7, 9, 11 and 13) pmoles of indicated Set1Cs.

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