Figure 2 | Cell Discovery

Figure 2

From: Binding to RNA regulates Set1 function

Figure 2

Set1C RNA binding requires dRRM, the N-SET domain, and Spp1. (a) A schematic representation of Set1, Set1YF/AA and Set1 deletion mutants with predicted RRM1, RRM2, N-SET and post-SET (hatched box) domains and associated subunits. (b) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and Coomassie blue staining of purified Set1Cs reconstituted with baculoviruses expressing FLAG-Set1 or FLAG-Set1 fragments and untagged subunits. (c, d) Radiolabeled GAL1 (c) and GAL10 (d) transcripts were subjected to in vitro RNA electrophoretic mobility shift assays with indicated Set1Cs. (e) Binding of Set1C and Set1C lacking Spp1 to radiolabeled GAL10. Set1C containing Set1∆N-SET is also shown.

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