Figure 3 | Cell Discovery

Figure 3

From: Binding to RNA regulates Set1 function

Figure 3

RRM but not N-SET regulates the genome-wide occupancy of Set1. (a, b) W303 set1∆::TRP1 pRS415-nHTP-SET1 (and SET1 mutant forms) cells were grown in SC -TRP-LEU. Protein levels of Set1 (a) and methylated histone H3 (b) were verified by western blots using anti-Set1 mAb and anti-H3K4me1, me2, and me3 antibodies, respectively. A Rap1 loading control is shown. (c) Average enrichment profiles of PTH-Set1 and PTH-Set1 mutants in genes>1500 bp. Read counts were normalized to read counts per million of mapped reads. ChIP-seq experiments were performed with the anti-Set1 mAb from set1∆::TRP1 cells expressing PTH-Set1 and PTH-Set1 mutants from the pRS415-nHTP (grown in SD -TRP -LEU). (d) Normalized occupancy profiles of Set1 at the indicated genes. Graphs were normalized to 10 million mapped reads for each mutant.

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