Figure 3 | Cell Discovery

Figure 3

From: Dihydroartemisinin selectively inhibits PDGFRα-positive ovarian cancer growth and metastasis through inducing degradation of PDGFRα protein

Figure 3

PDGFRα mediates the DHA-induced suppression of cancer cell growth, the EMT and migration. (a) A2780 and OVCAR3 cells treated with control or different lentivirus-mediated PDGFRα shRNAs for 72 h. (b) Cell viability was detected in A2780 and OVCAR3 cells after knocking down of PDGFRα. (c) Cell migration was evaluated in A2780 cells with or without knockdown of PDGFRα. (d) SK-OV3 cells stably expressing PDGFRα were treated with DHA for 24 h. The expression of PDGFRα was detected by western blotting. (e, f) Cell viability (e) and migration (f) was detected in SK-OV3 cells overexpression of PDGFRα after incubation with DHA. (g) The expression of the EMT-related proteins were detected in A2780 or OVCAR3 cells after incubation with DHA for 24 h. (h) The expression of E-cadherin and twist in A2780 cell with knockdown of PDGFRα. (i) EMT-related proteins detected in the SK-OV3 (PDGFRα null) cells after a 24-h exposure to DHA. The data shown are representative of values from at least three independent experiments with similar results (means±s.e.m.; *P<0.05; **P<0.01; ***P<0.001). DHA, dihydroartemisinin; PDGFR, platelet-derived growth factor receptor; EMT, epithelial–mesenchymal transition.

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