Figure 1
From: Tumor-targeted delivery of biologically active TRAIL protein
E. coli DH5α specifically resides in tumors. (a) Bacteria in tumors can be detected as early as 18 h after injection of 1 × 108 c.f.u. E. coli DH5α through the tail vein (left, B16-F10 murine melanoma in BALB/c mice; middle, H460 human lung carcinoma in nude mice; right, B16-F10 murine lung metastases in nude mice). (b) Tissue distribution of E. coli DH5α in immunocompetent BALB/c mice and immunocompromised nude mice using IVIS at different time points. Bars: photons/second (c) Various organs and tumors were removed from the mice and imaged using the IVIS without any processing at 30 min and day 4 after bacteria injection. (d) At different time points after infection, mice were killed. The livers, spleens and tumors were excised, homogenized and assayed for bacterial titers. ND, not detectable. Each value represents mean±s.d. from 10 mice. Photon collection was for 3 min at different time points after bacteria injection using the Xenogen IVIS 50 imaging system. C.f.u., colony-forming units; IVIS, In Vitro Imaging System.
