Figure 4
Expression of the immunodominant HA prevents the development of a broader response to additional tumor antigens. (a) Six-week-old BALB/c mice were depleted of Tregs by a single injection of 125 μg PC61 and subsequently injected s.c. with 5 × 105 AB1-HA tumor cells as in Figure 1. Mice that had rejected the first tumor and survived were rechallenged s.c. 2 months later with 5 × 105 AB1 (gray line) or AB1-HA (black line) or 1 × 105 4T1 (hatched gray line) or 4T1-HA (hatched black line) tumor cells (n=3 in each group). Graphs show top, the Kaplan–Meyer survival curves and bottom, the tumor volume growth curves (mm3). (b) Six-week-old BALB/c mice were depleted of Tregs by a single injection of 125 μg PC61 at day −1 and subsequently injected s.c. with 5 × 105 AB1-HA (black line) or AB1 (gray line) tumor cells at day 0 (n=3 in each group). Mice that had rejected the first tumor and survived were rechallenged 2 months later with 105 4T1 tumor cells, as well as age-matched naive mice as a control (n=2, not shown). Graph shows the tumor volume growth curves (mm3).
