Figure 5
In vitro effects of anti-herpes simplex virus type 1 (HSV-1) antibody on HF-green fluorescent protein (GFP). SKOV3 cells were plated for 24 h, and HF-GFP-infected carrier cells (a, b) or HF-GFP (c, d) were added to the media containing control serum (a, c) or anti-HSV-1 antiserum (b, d). Anti-HSV-1 antiserum was added to the media to give a final dilution of 1:50. Representative cytopathogenic effect (CPE) images are taken at 24 h using the Leica M205FA fluorescence stereomicroscope with a standard GFP filter set. SKOV3 cells were then fixed, stained with 0.2% crystal violet solution and observed at 30 h. The number of plaques was counted and expressed as a percentage of number obtained in control cultures (e).
