Figure 1
Cytotoxic activity of CTL expressing anti-flk1 cTCR. CTL were purified from C57BL/6 or BALB/c mice and transduced with anti-flk1 cTCR or anti-flk1 scFv-expressing retroviral vectors. (a) Forty-eight hours after gene transduction, the expression level of anti-flk1 scFv in CTL was analyzed by flow cytometry. Filled histogram represents the control; the dashed line, scFv–CTL; solid line, cTCR–CTL. (b) Anti-flk1 cTCR or anti-flk1 scFv-expressing CTL (cTCR–CTL or scFv–CTL) as effector cells were co-cultured with MS1 cells or B16BL6 cells as target cells at varying effector-to-target cell ratios. Four hours later, the cytotoxic activity of cTCR–CTL or scFv–CTL was determined using a standard 51Cr-release assay. Each point represents the mean±s.d. of three independent cultures.
