Figure 3 | Cancer Gene Therapy

Figure 3

From: Additive antileukemia effects by GFI1B- and BCR–ABL-specific siRNA in advanced phase chronic myeloid leukemic cells

Figure 3

BCR–ABL and GFI1B gene expression determined by real-time RT-PCR 48 h after siRNA transfection of primary advanced CML cells. (a) Forty-eight hours after silencing with both siRNAs in primary cells, the BCR–ABL expression decreased significantly (P<0.001). (b) Forty-eight hours after silencing with both siRNAs in primary cells, the GFI1B expression decreased significantly (P<0.001). Mean and s.d. are presented. Gene expression normalized to GAPDH by real-time RT-PCR. Control was set to 100%. BCR–ABL, breakpoint cluster region–Abelson murine leukemia oncogene; CML, chronic myeloid leukemia; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse transcription-polymerase chain reaction; siRNA, small interfering RNA.

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