Table 2 BCR–ABL and GFI1B genes expression determined by real-time RT-PCR 48 h after siRNA transfection in K562 cells

From: Additive antileukemia effects by GFI1B- and BCR–ABL-specific siRNA in advanced phase chronic myeloid leukemic cells

Treatment in K562 cells

BCR–ABL gene expression in % after transfection

P-value

GFI1B gene expression in % after transfection

P-value

Control (mock)

162±31

 

142±52

 

BCR–ABL siRNA (54 pM)

62±9*

<0.0001

133±27*

 

GFI1B siRNA (175 pM)

*135±24

<0.05

32±21

<0.0001

BCR–ABL + GFI1B siRNA

33±8

<0.0001

26±26

<0.0001

  1. Abbreviations: BCR–ABL, breakpoint cluster region–Abelson murine leukemia oncogene; RT-PCR, reverse transcription-polymerase chain reaction; siRNA, small interfering RNA.
  2. BCR–ABL gene and/or GFI1B gene expression were measured up to eightfold by real-time RT-PCR and results are shown as mean + s.d. normalized to GAPDH.
  3. *P<0.0001.
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