Figure 1
From: Toll-like receptor agonists shape the immune responses to a mannose receptor-targeted cancer vaccine
hMR-Tg and WT mice were injected s.c. at the tail base with 10 µg of B11-hCGβ. Local skin and inguinal LNs were collected 24 h later and cryopreserved. (a) hMR-mediated uptake of B11-hCGβ. IHC was performed with rabbit anti-hCGβ IgG, Dako EnVision kit and hematoxylin counterstain. The images were taken with a ×20 objective lens for skin and a ×4 objective lens for LN. The inserted cropped 40× image in the upper right corner of the Tg LN frame shows the morphology of representative hCGβ-positive cells in the LN. (b) Colocalization of hCGβ and hMR. Immunofluorescence dual staining was performed with rabbit anti-hCGβ and FITC-labeled mouse anti-hMR and followed by donkey anti-rabbit IgG-Cy3 and goat anti-FITC/Oregon green-Alexa488. The images were taken using a confocal microscope. (c) Colocalization of hCGβ and MHC class II. Immunofluorescence dual staining was performed with rabbit anti-hCGβ and biotinylated anti-mouse I-Ab and followed by goat anti-rabbit IgG-Alexa488 and strepavidin-Cy3. The images were taken using a confocal microscope. hCGβ, human chorionic gonadotropin beta chain; hMR-Tg, human mannose receptor transgenic; IHC, immunohistochemical; LN, lymph node; WT, wild-type.
