Figure 6
From: Toll-like receptor agonists shape the immune responses to a mannose receptor-targeted cancer vaccine
MR-targeted vaccine alone induced Th1 but not Th2 unresponsiveness. hMR-Tg mice were immunized s.c. in the paws with one or two doses of 10 µg of B11-hCGβ at a 26-day interval in the absence or presence of 20 µg of poly-ICLC and 25 µg of CpG as labeled for the following groups: G1: prime with B11-hCGβ alone; G2: prime with B11-hCGβ+poly-ICLC+CpG; G3: prime with B11-hCGβ, boost with B11-hCGβ+poly-ICLC+CpG; G4: prime with B11-hCGβ+poly-ICLC+CpG, boost with B11-hCGβ; G5: prime and boost with B11-hCGβ+CpG+poly-ICLC. Blood and spleens were collected 7 days after the first or second injection. Means±s.d. of (a) hCGβ-specific IFN-γ-ELISPOT numbers and (b) IgG1 and IgG2c titers from three mice per treatment are shown. Mice receiving a single immunization (G1 and G2) did not generate titers and are not included in (b). Student's t-test: ***P<0.001 compared G3 or G5 with G4. Similar results were obtained from repeated experiments. hCGβ, human chorionic gonadotropin beta chain; hMR-Tg, human mannose receptor transgenic; s.c., subcutaneously; WT, wild-type.
