Figure 3
From: miR-146a negatively regulates NK cell functions via STAT1 signaling
miR-146a expression is regulated by cytokines associated with NK cell activation. (a) Primary NK cells were treated with IL-10 (30 ng/ml), TGF-β (30 ng/ml) and IL-6 (30 ng/ml).33 After 4 h, miR-146a expression was quantified by qPCR. (b) Primary NK cells were stimulated with poly(I:C) (500 IU/ml), IL-12 (20 ng/ml), IL-15 (20 ng/ml) and IFN-α (1,000 IU/ml).8, 24, 34 After 12 h, miR-146a expression was quantified by qPCR. Data are representative of three independent experiments, statistical significance was determined as *P<0.05 and **P<0.01 compared with the control. Ctrl, control; IL, interleukin; INF, interferon; miR, microRNA; NS, nonsignificant; NK, natural killer; TGF, transforming growth factor; qPCR, quantitative PCR.
