Figure 4
From: miR-146a negatively regulates NK cell functions via STAT1 signaling
miR-146a targets STAT1 in NK cells. The protein expression of IRAK1, TRAF6 and STAT1 in (a) miR-146a-overexpressing NK92 cells and (b) miR-146a-inhibited NK-92 cells was measured by western blot. (c) Primary NK cells were transduced with miR-146a overexpression lentivirus; 24 h later, the expression of STAT1 was detected by western blot. (d) STAT1 expression of the mixed NK cells from CHB patients (n=8) or HCC patients (n=8) was detected. Data are representative of three independent experiments, statistical significance was determined as *P<0.05 and **P<0.01 compared with the control. CHB, chronic hepatitis B; HCC, hepatic carcinoma; miR, microRNA; NS, nonsignificant; NK, natural killer.
