Figure 5

STAT1 signaling contributes to NK cell cytotoxic activity. NK-92 cells (a) or primary NK cells (c) were treated with IFN-α (1000 IU/ml), fludarabine (50 μM; Flu) or both for 24 h; then, the cytotoxicity of these NK cells against HepG2 cells was analyzed. NK92-146a-over cells (b) or primary NK cells transduced with miR-146a overexpression lentivirus for 24 h (d) were treated with or without fludarabine, and then the cytotoxicity was detected. (e) NK cells from patients of CHB (n=10) and HCC (n=8) were transduced with has-STAT1-ORF lentivirus; 24 h later, the cytotoxicity was detected. Data are representative of three independent experiments, statistical significance was determined as *P<0.05 and ***P<0.001. CHB, chronic hepatitis B; HCC, hepatic carcinoma; INF, interferon; miR, microRNA; NK, natural killer.