Figure 5

C5a enhances glomerular IC deposition in FH^−/− mice with CSS. Representative glomerular immunofluorescence staining for C3 (green) and IgG (red) is shown for control FH^−/− mice without CSS (A), FH^−/− mice with CSS (B), and FH^−/− mice with CSS receiving C5aR_ant (C) or CP_inh (D). Linear glomerular capillary wall C3 staining typical for unmanipulated FH^−/− mice was present in all groups. Mesangial IgG was present in all groups with CSS (white asterisks) with some extension to the peripheral capillary walls (white arrows), but in relatively lesser amounts in FH^−/− mice treated with C5aR_ant (C) and greater amounts in those given CP_inh (D), in which there was significant extension to peripheral capillary walls (double white arrows). For purposes of localization, nuclei were stained with DAPI (blue) in D. Original magnifications, ×400.