Figure 1 | Cellular & Molecular Immunology

Figure 1

From: Differences in phenotype, homing properties and suppressive activities of regulatory T cells induced by epicutaneous, oral or sublingual immunotherapy in mice sensitized to peanut

Figure 1

Study design of experiments. (a) BALB/c mice were sensitized to peanut proteins. Then, the mice were treated by EPIT (100 μg), OIT (1 mg the first week, 2 mg the second week and 5 mg the five following weeks), SLIT (100 μg) or placebo (sham) for 8 weeks. Naive mice remained unsensitized and untreated. Following treatment, the blood was recovered for sIgE and sIgG2a measurement, and the mice were killed for spleen and lymph node recovery for cell culture and FACS analysis. (b) BALB/C mice were sensitized and treated as described above (donor mice). After treatment (n=15 for each group) or 8 weeks after the end of treatment (n=15 for each group), the donor mice were killed, the CD4+CD25+ T cells were sorted from spleen cells and transferred into peanut-sensitized non-treated mice. Three days after the transfer, the mice were submitted to a 10-day sustained oral exposure to peanuts. The day after the last challenge, the mice were killed for organ recovery for esophagus histology and spleen cell culture.

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