Figure 3 | Cellular & Molecular Immunology

Figure 3

From: Differences in phenotype, homing properties and suppressive activities of regulatory T cells induced by epicutaneous, oral or sublingual immunotherapy in mice sensitized to peanut

Figure 3

Different phenotypes of Tregs induced by EPIT, OIT or SLIT. Sensitized mice were EPIT-, OIT-, SLIT- or sham-treated for 8 weeks. At the end of treatment, the spleen cells were harvested for flow cytometry analysis. (a) Gating strategies: the cells were gated on CD4+ cells among the lymphocytes identified by FSC/SSC after doublet exclusion; the percentages of CD25+Foxp3+ (b), CD25+IL10+ (c) or LAP+ cells (d) and the percentages of CD25+Foxp3+CD44hiCD62L− or CD25+Foxp3+CD44loCD62L+ cells (e) were analyzed. (f) Using the same gating strategies, the cells were gated on CD4+CD25+Foxp3+ cells and the percentage of cells expressing CTLA-4 was analyzed. The data are shown as the median with range from three independent experiments with n=6 for each group of mice. *P<0.05; **P<0.01; ***P<0.001; #P<0.01 compared with EPIT.

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