Figure 3 | Cellular & Molecular Immunology

Figure 3

From: IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity

Figure 3

IL-25 alternatively activates macrophages (M2 polarization). (a, c) Immunofluorescent staining for F4/80 (macrophage marker) and ARG1 (M2 marker) in the eWAT (a) and liver (c). Images were representative of three independent experiments and photographed at × 40 magnification. Scale bar: 50 μm. (b, d) The mRNA expression of the macrophage recruiting molecule Mcp-1, the macrophage marker F4/80, the M2 markers Arg-1 and Ym1, the type-2 immune cytokine Il-13, the M1 marker Inos and the type-1 immune cytokine Tnfα in the eWAT (b) and liver (d) after IL-25 treatment. The data are presented as the mean±s.d. n=5; *P<0.05 versus the NCD group, #P < 0.05 versus the HFD group. (e, f) BMMCs were treated with IL-25 (50 ng/mL) for 72 h. The expression of M1/M2 markers (e), type-1/type-2 cytokines and transcription factors (f) were examined in BMMCs. The data are presented as the mean±s.d. n=3; *P<0.05 versus the Veh group. BMMC, bone marrow-derived macrophage; eWAT, epididymal white adipose tissue; HFD, high-fat diet; IL, interleukin; NCD, normal chow diet.

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