Figure 4
IL-25 enhances lipolysis in macrophages. (a) Lipid uptake in macrophages. Macrophages were able to take up more lipid droplets during treatment with IL-25 in the presence of OA (oleic acid). The ImageJ software was used for gray scanning. Gray histograms are presented as the mean±s.d. of three independent experiments. n=5; *P<0.05 versus the OA group. (b) The alteration of lipogenesis and lipolysis enzymes in RAW264.7 macrophages treated with cytokines. The left panel shows the typical western blot bands, and the right panel shows the quantitative analysis of the gray scan. The ImageJ software was used for gray scanning. *P<0.05 versus the Veh group. (c) Oil Red O staining of 3T3-L1 adipocytes treated with IL-25 or Mø-IL-25. The ImageJ software was used for gray scanning. The gray histograms are presented as the mean±s.d. of three independent experiments. n=5; *P<0.05 versus the Veh group, #P<0.05 versus the Mø group. (d, e) The alteration of the levels of lipolysis and lipogenesis enzymes in 3TL-L1 adipocytes treated with cytokines (d) or co-cultured with RAW264.7 macrophages treated with cytokines (e). The gray histograms are presented as the mean±s.d. of three independent experiments. n=5; *P<0.05 versus the Veh group. Different cytokines such as IL-25 (50 ng/ml), IFNγ (50 ng/ml) and LPS (10 ng/ml) were applied. LPS, lipopolysaccharide.
